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KMID : 0381919940240030067
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Korean Journal of Microscopy
1994 Volume.24 No. 3 p.67 ~ p.77
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An Electron-Microscopical Study of Cellulase Activity on Germinating Endosperm of Cannabis saiva L.
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Kim Young-Hee
Kim Eun-Soo
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Abstract
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Storage material of endosperm cells digested by various enzymes should be transported to the embryo. At this time, the cellulose of the endosperm cell wall is guessed to be hydrolyzed by the cellulase enabling to transfer the storage material from the endosperm cells to the embryo. Therefore, this study has been carried out to investigate the ultrastructure of endosperm and the localization of the cellase activity on Cannabis sativa L. during germination. Endosperm cells contain a large number of lipid bodies and protein bodies with globoids as the storage material. During gemination they are gradually degenerated, however, the former almost remain until the cells are completely digested. Electron-microscopical reaction products of cellulase on endosperm cells are present. The closer the embryo, the more amount of reaction products on the endosperm cell wall are appeared.
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KEYWORD
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